HOME - Clinical cases - Oral Hygiene & Prevention
 
 
07 November 2022

The importance of keeping your toothbrush clean

Gianna Maria Nardi


The health of the oral cavity is determined by a condition of eubiosis of the oral cavity, ensuring the microbiological balance of about 900 different microbial species. In the motivational approach to correct home oral hygiene, the sanitation of dedicated tools must be considered.  

Patients must be informed that during brushing, the bristles of the toothbrush and the interdental brushes and tongue cleaners are contaminated by bacterial biofilm, saliva, blood, food residue and residues of toothpaste or gel.  

Another potential health hazard for the toothbrush is environmental contamination. Several studies have evaluated the microbial contamination on the brush head and between the tufts of bristles, after one and three months of use in bathrooms. This highlights the presence of E. Coli on the surface of toothbrushes, probably also due to the nebulization of substances and microorganisms present in bathrooms.  

This scientific evidence has focused on exposure triggered by fecal microorganisms and E. Coli, a gram-negative microorganism belonging to the Enterobacteriaceae group. It is gram negative, facultative anaerobe, ferments glucose, reduces nitrate and is catalase-positive and oxidase-negative. These microorganisms have a broad spectrum of virulence with two important factors:

  • Specialized adhesins that allow it to remain in the gastrointestinal tract or urinary tract
  • Exotoxins that produce heat stable toxins, Hemolysin Hya.

In the gastrointestinal tract it can mainly cause septicemia, neonatal meningitis, urinary tract infections and gastroenteritis.

Furthermore, research studies have highlighted the presence of other bacterial species including: Streptococcus Mutans, Staphylococcus Aureus, Pseudomonas, Candida and Lactobacillus. Controls at 24, 48 and 72 hours showed higher contamination than toothbrushes covered by a case. 

The hygiene of toothbrushes and other oral hygiene devices must be treated with the importance it deserves for a reduction of the risk of onset of oral cavity and systemic diseases. 

To facilitate the management of sanitizing the toothbrush at least twice a week, people may use spray solutions to protect brushes and devices from bacteria, fungi and viruses. Some studies have found that soaking your toothbrush in a 3% hydrogen peroxide solution or antibacterial mouthwash can help kill any bacteria that may be on it, according to an Oct. 2022 article on WebMD. Soaking a toothbrush in white vinegar once a week may also help disinfect it. The sprays should be used a few inches away from the surface to properly sanitize the toothbrush.

Ilkay Peker,Gulcin Akca,Cigdem Sarikir,Meryem Toraman Alkurt, Irem Celik. "Effectiveness of alternative methods for toothbrush disinfection: an in vitro study." Scientific World Journal; 2014:726190..doi:10.1155/2014/726190

A Misirligil, N Saygun, Z Misirligil. "Effects of storage conditions on the rate of disappearance of bacterial contamination of toothbrushes." Mikrobiyol Bul. 1984 Jan:18(1):47-52

Related articles

A large epidemiological health investigation, the Helsinki Ageing Study (HAS), was performed in 1989–1991 in Helsinki, Finland.


This paper presents a literature review of oral hygiene care for adults with dementia in residential aged care facilities


To conduct a systematic review of randomised trials assessing the association between personal oral hygiene and dental caries in the absence of the confounding effects of fluoride.


The aim of our study was to qualitatively and quantitatively synthesize the evidence regarding ways to improve motivation of oral hygiene among orthodontic patients


The aims of this study were to examine self-reported oral hygiene habits and oral health problems of a sample of adult Kuwaitis


Read more

Medit, a global leader in digital dentistry solutions, today announced the launch of Medit Aligners, a premium digital clear aligner solution designed to deliver faster turnaround times and more...


News     19 September 2025

UK Dental Market Trends Revealed

Specialist business property adviser, Christie & Co, has launched its Dental Market Review 2025 report, which offers a panoramic view of the UK dental business sector, spotlighting important...


News     19 September 2025

USOSM Supports OMS Foundation Research

U.S. Oral Surgery Management (USOSM) is proud to announce its continued support of the OMS Foundation, a 501(c)(3) nonprofit that strives to improve the quality and safety of patient care by fueling...


n this study we investigated the accuracy of diagnoses of oral mucosal diseases made by family physicians (without a dental degree), other categories of physicians, and general dental practitioners...


Editorials     19 September 2025

Cooking in the Name of Health

Students from across the health sciences gained nutrition know-how and culinary chops in a unique new course


 
 
 
 

 
 
 
 

Most popular

 
 

Events