HOME - Clinical cases - Digital Dentistry
10 March 2020

CAD/CAM materials might reduce gingival inflammation during provisional phase in prosthodontics rehabilitation

Massimo Gagliani

Several prosthodontics rehabilitation processes are needed to recreate a correct masticatory function. The provisional stage is quite important and materials employed in this phase are not completely satisfactory, not only for resistance but also for the interaction with dental plaque and soft tissues.
A very interesting study was published on one of the most important journal in dental research.
The aim of this study was to evaluate the cytotoxicity and inflammatory response of different types of provisional restorative traditional and CAD/CAM materials to mice gingival fibroblasts.

The Authors choose to investigate cytotoxicity of provisional material discs :
-thermal-polymerized acrylic resin
-auto-polymerized acrylic resin
-bisacrylic resin
-nano-ceramic resin for CAD/CAM
-prefabricated polymer block for CAD/CAM

All these disks were tested to Mice (Balb/c) gingival cell both in direct and indirect contact (extracts) at 24, 48 and 72 h, using the MTT and Alamar blue assays. Materials extracts (24 h) were applied to the cell culture (indirect contact) or cells were seeded on discs of provisional materials, and the cytotoxicity and production of IL-6, IL-1β and TNF-α after 24, 48 and 72 h were analyzed through MTT, Alamar Blue® and ELISA. Culture medium was used as control for indirect contact assay (extract) and the surfaces of the wells without discs of provisional materials were used as control for direct contact assay. 

The results were quite clear: Auto-polymerized and bisacrylic resins (direct contact) reduced cell viability after 24, 48 and 72 h compared to control (P < .05). In addition, Auto-polymerized and bisacrylic resins increased IL-6, IL-1β and TNF-α levels mainly at 24 h when compared to the other materials (P < .001). Indirect contact (extract) was not cytotoxic to cells at all periods compared to control (P > .05).

Auto-polymerized and bisacrylic resins were more cytotoxic to mice gingival fibroblasts. CAD/CAM nano ceramic resin and prefabricated polymer blocks are more predictable materials to preserve the periodontal soft tissues during the provisional phase of a prosthodontics rehabilitation.

For additional informations: Cytotoxicity and inflammatory response of different types of provisional restorative materials.

Related articles

CAD/CAM technology has been used in industry for long time and has increased in popularity over the past years in dentistry from making impressions, casts, provisional and final restorations. The...

Inlay and overlay are a good and right choice in case of large carious and restorative defects. The introduction of CAD/CAM system gives the possibilities to...

Premolars are the most difficult elements to restore and have a high failure rate due to their anatomical position and the forces they undergo. Therefore the choice of the material to restore...

Contemporary computer-aided design and manufacturing (CAD/CAM) systems involve a wide range of dental restoration indications such as inlays, onlays, laminate...

With the development of CAD/CAM system, nowadays the most used materials in the prosthetic fields are: zirconia and lithium disilicate. In particular, in a...

Read more

Many times primary teeth are given less importance as they are believed to shed automatically. Quite the opposite, primary teeth help in chewing, speech, are...

Platelet-rich plasma (PRP)  is a concentration of platelets derived from the centrifugation of autologous whole blood. It is extensively used to promote soft...

The use of zirconia in restorative dentistry has grown exponentially over the past decade. Early zirconia formulations were used for frameworks because of...

Co-authors: A. Comba, F. Florenzano

The increasing demand for beautiful and harmonious smiles has greatly increased the use of ceramic laminates. These esthetic restorations, that are claimed to...

Several milestone papers have been written in the last years on Endodontic Retreatments and some of the most important among them have been authored by Prof....




Most popular